BufferPrep
By using BufferPrep, you acknowledge the site’s Terms of Service and Scientific Disclaimer and agree to verify all results before laboratory use.

Scientific use note

  • What this tool does: scales common fixed buffer recipes, calculates stock dilution volumes, or calculates mass from molarity, volume, and molecular weight.
  • What this tool does not do: replace institutional protocols, validate sterility, provide clinical or manufacturing-grade validation, calculate arbitrary pH recipes in v1, or replace SDS/MSDS review.
  • Research-use reference only. Verify all calculations against your institutional protocol before use. Check SDS/MSDS documentation for each reagent and follow standard laboratory PPE, handling, and waste-disposal procedures.
  • Stock concentration must be greater than the target concentration for a valid dilution.

Stock dilution calculator

Use C1V1 = C2V2 to calculate how much stock solution and diluent you need for a target working solution.

Results

Volume of stock solution to use
50.0 mL
Volume of diluent to add
450 mL

Step-by-step calculation