Saline buffer
PBS
Scale a common 10X PBS formulation for bench preparation and dilute to working concentration as needed.
- Base concentration
- 10X
- Common use
- Saline buffer
- pH
- 7.4
Buffer recipes
Search common formulations, compare base concentrations, and open a dedicated calculator for each recipe.
Electrophoresis buffer
TAE
Scale a common 50X TAE stock recipe for electrophoresis workflows.
- Base concentration
- 50X
- Common use
- Electrophoresis buffer
- pH
- 8.3
Electrophoresis buffer
TBE
Scale a common 10X TBE stock recipe for electrophoresis applications.
- Base concentration
- 10X
- Common use
- Electrophoresis buffer
- pH
- 8.3
Storage buffer
TE
Prepare a common 1X TE buffer using Tris and EDTA stock references.
- Base concentration
- 1X
- Common use
- Storage buffer
- pH
- 8
Wash buffer
TBS
Scale a common 10X TBS formulation for blotting and wash workflows.
- Base concentration
- 10X
- Common use
- Wash buffer
- pH
- 7.6
Wash buffer
TBST
Scale a common 1X TBST wash buffer for immunoblotting workflows.
- Base concentration
- 1X
- Common use
- Wash buffer
- pH
- 7.6
Hybridization buffer
20X SSC
Scale a common 20X SSC stock recipe for nucleic acid hybridization workflows.
- Base concentration
- 20X
- Common use
- Hybridization buffer
- pH
- 7
Stock solution
0.5 M EDTA
Prepare a common EDTA stock solution with clear pH adjustment guidance and hydrate-sensitive warnings.
- Base concentration
- Reference stock
- Common use
- Stock solution
- pH
- 8
Stock solution
1 M Tris-HCl
Calculate the mass of Tris base for a common 1 M Tris-HCl stock and follow your validated pH adjustment procedure.
- Base concentration
- Reference stock
- Common use
- Stock solution
- pH
- 8
Stock solution
1 M Tris Base
Calculate Tris base mass for a 1 M stock solution without pH adjustment assumptions.
- Base concentration
- Reference stock
- Common use
- Stock solution
- pH
- Verify with protocol