BufferPrep
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Electrophoresis buffer

Tris-Acetate-EDTA (TAE) Calculator

Scale a common 50X TAE stock recipe for electrophoresis workflows.

Scientific use note

  • What this tool does: scales common fixed buffer recipes, calculates stock dilution volumes, or calculates mass from molarity, volume, and molecular weight.
  • What this tool does not do: replace institutional protocols, validate sterility, provide clinical or manufacturing-grade validation, calculate arbitrary pH recipes in v1, or replace SDS/MSDS review.
  • Research-use reference only. Verify all calculations against your institutional protocol before use. Check SDS/MSDS documentation for each reagent and follow standard laboratory PPE, handling, and waste-disposal procedures.
  • This page presents static recipe information in the initial HTML and adds local interactive scaling in the browser.
  • Adjust pH slowly using acid or base while measuring with a calibrated pH meter. Follow your lab protocol.

Recipe calculator

Scale this 50X formulation locally in your browser. Results update without page reload and stay on your device.

Current target: 1X, 500 mL

Base recipe: 50X

Reagent table

Updates instantly as inputs change
Component Type Amount Notes
Tris solid 2.42 g Use your lab's standard reagent grade.
Acetic acid liquid 0.571 mL Use your lab's standard reagent grade.
0.5 M EDTA stock solution 1.00 mL Use your lab's standard reagent grade.

Bench protocol

  1. 1.Add roughly 70% of the final volume of water to a suitable container. Prepare 1X TAE for a final batch of 500 mL.
  2. 2.Dissolve the Tris base before adding glacial acetic acid and EDTA stock.
  3. 3.Mix thoroughly and verify the formulation against your lab protocol.
  4. 4.Bring to final volume with water and mix again.
  5. 5.Dilute the stock further for working solutions as required by your application.

Recipe notes

  • Common formulation; verify against your institutional protocol before use.
  • Common formulation; verify against your institutional protocol before use.

Questions or correction requests can be sent to Email Us.

FAQ

Is this a working-strength TAE recipe?

The stored base recipe is a common 50X stock. Use the concentration selector to scale to working strength when needed.

Should I verify this formulation?

Yes. TAE composition can vary slightly across labs, so verify against your institutional protocol before use.