BufferPrep
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Storage buffer

TE Buffer Calculator

Prepare a common 1X TE buffer using Tris and EDTA stock references.

Scientific use note

  • What this tool does: scales common fixed buffer recipes, calculates stock dilution volumes, or calculates mass from molarity, volume, and molecular weight.
  • What this tool does not do: replace institutional protocols, validate sterility, provide clinical or manufacturing-grade validation, calculate arbitrary pH recipes in v1, or replace SDS/MSDS review.
  • Research-use reference only. Verify all calculations against your institutional protocol before use. Check SDS/MSDS documentation for each reagent and follow standard laboratory PPE, handling, and waste-disposal procedures.
  • This page presents static recipe information in the initial HTML and adds local interactive scaling in the browser.
  • Adjust pH slowly using acid or base while measuring with a calibrated pH meter. Follow your lab protocol.

Recipe calculator

Scale this 1X formulation locally in your browser. Results update without page reload and stay on your device.

Current target: 1X, 500 mL

Base recipe: 1X

Reagent table

Updates instantly as inputs change
Component Type Amount Notes
1 M Tris-HCl stock solution 5.00 mL Use your lab's standard reagent grade.
0.5 M EDTA stock solution 1.00 mL Use your lab's standard reagent grade.

Bench protocol

  1. 1.Add most of the final water volume to a clean container. Prepare 1X TE for a final batch of 500 mL.
  2. 2.Add Tris-HCl stock and EDTA stock in the listed amounts.
  3. 3.Mix thoroughly and bring to the final volume with water.
  4. 4.Use sterile technique if the buffer will be used for nuclease-sensitive workflows.
  5. 5.Label with concentration, pH, and date according to your lab practice.

Recipe notes

  • Common formulation; verify against your institutional protocol before use.

Questions or correction requests can be sent to Email Us.

FAQ

Is this TE recipe based on stock solutions?

Yes. This version uses 1 M Tris-HCl and 0.5 M EDTA stock solutions for a common 1X TE formulation.

Can TE formulations vary?

Yes. Verify the final concentration and pH against your laboratory protocol before preparation.