Wash buffer

Tris-Buffered Saline with Tween 20 (TBST) Calculator

Scale a common 1X TBST wash buffer for immunoblotting workflows.

Scientific use note

What this tool does: scales common fixed buffer recipes, calculates stock dilution volumes, or calculates mass from molarity, volume, and molecular weight.
What this tool does not do: replace institutional protocols, validate sterility, provide clinical or manufacturing-grade validation, calculate arbitrary pH recipes in v1, or replace SDS/MSDS review.
Research-use reference only. Verify all calculations against your institutional protocol before use. Check SDS/MSDS documentation for each reagent and follow standard laboratory PPE, handling, and waste-disposal procedures.
This page presents static recipe information in the initial HTML and adds local interactive scaling in the browser.
Adjust pH slowly using acid or base while measuring with a calibrated pH meter. Follow your lab protocol.

Scale this 1X formulation locally in your browser. Results update without page reload and stay on your device.

Final volume

Volume unit

Final concentration

Significant figures

Current target: 1X, 500 mL

Base recipe: 1X

Updates instantly as inputs change

Component	Type	Amount	Notes
Tris	solid	1.21 g	Use your lab's standard reagent grade.
NaCl	solid	4.00 g	Use your lab's standard reagent grade.
Tween 20	liquid	0.500 mL	Equivalent to 0.1% v/v in a 1 L final volume.

1.Add most of the final water volume to a clean beaker or bottle. Prepare 1X TBST for a final batch of 500 mL.
2.Dissolve Tris base and sodium chloride completely.
3.Adjust pH with a calibrated meter if your protocol requires it.
4.Add Tween 20 carefully and mix without excessive foaming.
5.Bring to final volume with water and invert to homogenize.

Questions or correction requests can be sent to Email Us.

This page uses a common 1X TBST formulation. Verify detergent concentration against your assay protocol.

Dissolve salts first, then add Tween 20 and mix gently to reduce foaming.